RECOMBINANT HUMAN INSULIN .2. SIZE-EXCLUSION HPLC OF BIOTECHNOLOGICALPRECURSORS - FACTORS INFLUENCING ON RETENTION AND SELECTIVITY

Authors
KLYUSHNICHENKO VE WULFSON AN
Citation
Ve. Klyushnichenko et An. Wulfson, RECOMBINANT HUMAN INSULIN .2. SIZE-EXCLUSION HPLC OF BIOTECHNOLOGICALPRECURSORS - FACTORS INFLUENCING ON RETENTION AND SELECTIVITY, Pure and applied chemistry, 65(10), 1993, pp. 2265-2272
Citations number
56
Categorie Soggetti
Chemistry
Journal title
Pure and applied chemistry → ACNP
ISSN journal
00334545
Volume
65
Issue
10
Year of publication
1993
Pages
2265 - 2272
Database
ISI
SICI code
0033-4545(1993)65:10<2265:RHI.SH>2.0.ZU;2-3
Abstract
The mechanism of exclusion-sorption interaction of insulin-containing proteins with the column support in accordance with the chemical and t hree-dimensional structure in native and denaturating conditions was o bserved. The mechanisms of weak adsorption of linear proinsulin and fu sion protein presenting in native, and dynamics of formation SDS-prote in complex in denaturating conditions have been revealed. The obtained results are used for SE HPLC analysis of the main and intermediate pr oducts practically at all steps of recombinant human insulin productio n technology.