Citation
W. Blum et al., RAPID CHARACTERIZATION OF ARTEMETHER AND ITS IN-VITRO METABOLITES ON INCUBATION WITH BOVINE HEMOGLOBIN, RAT-BLOOD AND DOG-BLOOD BY CAPILLARY GAS-CHROMATOGRAPHY CHEMICAL-IONIZATION MASS-SPECTROMETRY, Journal of chromatography B. Biomedical sciences and applications, 710(1-2), 1998, pp. 101-113
Citations number
41
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273
→ ACNP
Volume
710
Issue
1-2
Year of publication
1998
Pages
101 - 113
Database
ISI
SICI code
0378-4347(1998)710:1-2<101:RCOAAI>2.0.ZU;2-G
Abstract
A fast and sensitive analytical method was developed to characterize a
rtemether and its metabolites in small amounts in body fluids. The ext
racts were derivatized with N-methyl-N-trimethylsilyltrifluoroacetamid
e, separated on an optimized capillary gas chromatographic system and
identified by chemical ionization mass spectrometry by using ammonia a
s reagent gas. The analytical assay is demonstrated on samples extract
ed from bovine hemoglobin, rat blood and dog blood. Full mass spectra
of artemether and three metabolites were obtained at a level of 1.10(-
6) g/ml. (C) 1998 Elsevier Science B.V. All rights reserved.