Citation
Ec. Sekhar et al., DETERMINATION OF TERAZOSIN IN HUMAN PLASMA, USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION, Journal of chromatography B. Biomedical sciences and applications, 710(1-2), 1998, pp. 137-142
Citations number
24
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273
→ ACNP
Volume
710
Issue
1-2
Year of publication
1998
Pages
137 - 142
Database
ISI
SICI code
0378-4347(1998)710:1-2<137:DOTIHP>2.0.ZU;2-X
Abstract
A selective, sensitive, rapid and reproducible high-performance liquid
chromatographic method for the determination of terazosin in plasma i
s described. The structurally related compound prazosin was used as an
internal standard. The method comprises extraction with methylene chl
oride followed by chromatography on a C-18 reversed-phase column. The
compounds were detected using spectrofluorimetry. The absolute recover
ies were more than 90% with a minimal detection of 1 ng/ml and calibra
tion curve was linear between 1 and 80 ng/ml. (C) 1998 Elsevier Scienc
e B.V. All rights reserved.