DETERMINATION OF VISNAGIN IN AMMI-VISNAGA HAIRY ROOT CULTURES USING SOLID-PHASE EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A high-performance liquid chromatographic method was developed for sep aration of the furochromone fraction and for determination of visnagin in Ammi visnaga hairy root cultures. Lyophilized samples were extract ed with chloroform:methanol (1:1, v/v) and purified on solid-phase ext raction cartridges. HPLC analyses were performed on a Eurospher 100-C- 8 Knauer column and the mobile phase was 29:28:526:417 (v/v/v/v) aceto nitrile:tetrahydrofuran:30 mM citric acid (pH 3.0):methanol. Quercetin was used as internal standard. Peaks were identified by addition of a uthentic standards and/or by diode-array detection. (C) 1998 Academic Press.