Je. Atwater et al., DISSOLVED-OXYGEN DETERMINATION BY ELECTROCATALYSED CHEMILUMINESCENCE WITH IN-LINE SOLID-PHASE MEDIA, Journal of bioluminescence and chemiluminescence, 13(3), 1998, pp. 125-130
Dissolved elemental oxygen is determined in a flowing aqueous stream u
sing glucose oxidase to catalyse the reaction between D-glucose and O-
2 to produce hydrogen peroxide. The revels of the resulting H2O2 are d
etected and quantified by luminol chemiluminescence using in-line soli
d phase media for pH adjustment of the reagent stream and for controll
ed release of the luminophore. The reaction is initiated by electroche
mical catalysis. By the use of excess D-glucose in the reagent flow st
ream, the intensity of chemiluminescence is rendered proportional only
to fluctuations in the dissolved O-2 concentration. The methodology p
rovides a means for the detection of aqueous O-2 in the range 0-10 mg/
L. (C) 1998 John Wiley & Sons, Ltd.