Jg. Vanderbeek et al., GENETIC-VARIATION AMONG PARTHENOGENETIC MELOIDOGYNE SPECIES REVEALED BY AFLPS AND 2D-PROTEIN ELECTROPHORESIS CONTRASTED TO MORPHOLOGY, Fundamental and applied nematology, 21(4), 1998, pp. 401-411
Isolates of the ameiotic parthenogenetic species Meloidogyne are?zaria
, M. hapla race B, M. incognita, and M. java nica and of the meiotic p
arthenogenetic species M. chitwoodi, M. fallax, M. hapla race A, and M
. naasi were compared for total soluble proteins using two-dimensional
gel electrophoresis (2-DGE), total genomic DNA using electrophoresis
of amplified fragment length polymorphisms (AFLP), and morphological c
haracters by morphometric measurements and from literature. Data sets
were converted to similarity coefficients using the Dice coefficient,
based on more than 100 protein spots, 192 AFLP fragments, and 21 morph
ological characters. UPGMA dendrograms based on protein and DNA data w
ere congruent: with both types of data, M. hapla race A and race B clu
stered together, the three tropical species M. incognita, M. javanica,
and M. arenaria formed another cluster, and the species specialized o
n Gramineae -M. naasi, M. chitwoodi, and M. fallax- were distant from
the rest, with high similarity between M. chitwoodi and M. fallax. The
dendrogram for morphological data was different from the dendrograms
for molecular data, particularly for M. incognita and M. naasi. The re
asons for this discrepancy between protein and DNA studies on the one
hand and morphological studies on the other hand are discussed. (C) Or
stom/Elsevier, Paris.