Lz. Song et E. Gouaux, CRYSTALLIZATION OF THE ALPHA-HEMOLYSIN HEPTAMER SOLUBILIZED IN DECYLDIMETHYLPHOSPHINE AND DECYLDIETHYLPHOSPHINE OXIDE, Acta crystallographica. Section D, Biological crystallography, 54, 1998, pp. 276-278
Citations number
26
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biophysics,Biology
Crystals of the alpha-hemolysin heptamer, a transmembrane pore-forming
toxin from Staphylococcus aureus, have been grown using the nonionic
detergents n-decyldimethyl- and n-decyldiethylphosphine oxide, phospho
rus homologs of the ionic amine oxide detergents. Five crystal forms w
ere obtained, one of which diffracted X-rays to 3 Angstrom resolution.
This crystal form displayed elements of pseudo mm symmetry in screene
d precession photographs yet it was triclinic with unit-cell dimension
s a = 173, b = 173, c = 102 Angstrom, alpha = 92.6, beta = 94.8, gamma
= 90.2 degrees.