CRYSTALLIZATION OF THE ALPHA-HEMOLYSIN HEPTAMER SOLUBILIZED IN DECYLDIMETHYLPHOSPHINE AND DECYLDIETHYLPHOSPHINE OXIDE

Crystals of the alpha-hemolysin heptamer, a transmembrane pore-forming toxin from Staphylococcus aureus, have been grown using the nonionic detergents n-decyldimethyl- and n-decyldiethylphosphine oxide, phospho rus homologs of the ionic amine oxide detergents. Five crystal forms w ere obtained, one of which diffracted X-rays to 3 Angstrom resolution. This crystal form displayed elements of pseudo mm symmetry in screene d precession photographs yet it was triclinic with unit-cell dimension s a = 173, b = 173, c = 102 Angstrom, alpha = 92.6, beta = 94.8, gamma = 90.2 degrees.