BEHAVIOR OF SPERMATOGONIA FOLLOWING RECOVERY FROM BUSULFAN TREATMENT IN THE RAT

This study describes the morphological behaviour of spermatogonia foll owing recovery from two doses of busulfan treatment in the rat. Twenty days after the second intraperitoneal injection of busulfan, the test es lost most of their spermatogenic cells and there were fewer dispers ed singly surviving spermatogonia. These surviving cells were in close contact with the basal portions of adjacent Sertoli cells and the shr unken basal lamina, and were the source for repopulating the depleted seminiferous epithelium. During the initial stage of repopulation (48 days later), surviving spermatogonia underwent a phase of active proli feration: type A spermatogonia underwent symmetric and asymmetric divi sions; type B spermatogonia underwent asynchronous differentiation. At day 96, normal spermatogenesis was fully recovered in many seminifero us tubules, represented by 80% of the rats regaining various degrees o f fertility at day 120. These data provide an additional model for the study of self-renewal of stem spermatogonia and suggest that the asym metric division of type A spermatogonia and their close contact with b oth the basal lamina and the Sertoli cells may be involved in regulati ng the number of stem spermatogonia and the delicate process of normal spermatogenesis.