RESOLUTION OF FLUORINATED AMINOMETHANPHOSPHONIC ACIDS CATALYZED BY PENICILLIN-G ACYLASE-III

In this work, we have used penicillin G acylase (EC 3.5.1.11) from E. coli to resolve kinetically four fluorinated aminomethanphosphonic aci ds: 1: 1-trifluoromethyl-, 2:1-p-fluorophenyl-, 3:1-m-fluorophenyl-, 4 : 1-p-trifluoromethylphenyl-1-aminomethanphosphonic acids. This enzyme catalyses enantioselectively the hydrolysis of the N-phenylacetylated derivatives of 1-4, The enantiomeric excesses determined via P-31 NMR spectra of the diastereomeric Pd(II) complexes of 1-4 were high for e ach compound studied.