SIMPLIFICATION OF ADULT MOSQUITO BIOASSAYS THROUGH USE OF TIME-MORTALITY DETERMINATIONS IN GLASS BOTTLES

A simple method is described for treating 250-ml glass Wheaten bottles with insecticide, and using them as test chambers for detecting insec ticide resistance in mosquito and sandfly populations. The methods for treating bottles, obtaining baseline data, and applying this techniqu e to insects from the field are described. Sample data are presented f rom tests run on different vector species using a variety of insectici des. Time-mortality data from the bottle bioassay are presented alongs ide results from biochemical detection methods applied to the same mos quito population. The potential role, advantages, and limitations of t he time-mortality bottle method are discussed.