Ft. Vreede et al., SEQUENCE-INDEPENDENT AMPLIFICATION AND CLONING OF LARGE DSRNA VIRUS GENOME SEGMENTS BY POLY(DA)-OLIGONUCLEOTIDE LIGATION, Journal of virological methods, 72(2), 1998, pp. 243-247
Citations number
19
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
A strategy was developed for sequence-independent synthesis and amplif
ication of full-length cDNA of 3-4 kb genes of dsRNA viruses. The meth
od of single primer amplification (Lambden et al., 1992) was adapted b
y the inclusion of a 3' poly(A) tail to an oligonucleotide ligated to
dsRNA genome segments as a template for oligo(dT)-primed cDNA synthesi
s. Full-length copies of the largest genome segments, 1 (4 kb) and 2 (
3 kb), of African horst sickness virus (AHSV) have been cloned, termin
ally sequenced and expressed in vitro. (C) 1998 Elsevier Science B.V.
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