PROMOTION AND INHIBITION OF L-TYPE CA2-SUBUNIT( CHANNEL FACILITATION BY DISTINCT DOMAINS OF THE BETA)

Ca2+ current potentiation by conditioning depolarization is a general mechanism by which excitable cells can control the level of Ca2+ entry during repetitive depolarizations. Several types of Ca2+ channels are sensitive to conditioning depolarization, however, using clearly dist inguishable mechanisms. In the case of L-type Ca2+ channels, prepulse- induced current facilitation can only be recorded when the pore-formin g alpha(1C) subunit is coexpressed with the auxiliary beta(1), beta(3) , or beta(4), but not beta(2), subunit, These four beta subunits are c omposed of two conserved domains surrounded by central, N-terminal, an d C-terminal variable regions. Using different deleted and chimeric fo rms of the beta(1) and beta(2) subunits, we have mapped essential sequ ences for L-type Ca2+ channel facilitation. A first sequence, located in the second conserved domain of all beta subunits, is responsible fo r the promotion of current facilitation by the beta subunit. A second sequence of 16 amino acids, located on the N-terminal tail of the beta (2) subunit, induces a transferable block of L-type current facilitati on. Site-specific mutations reveal the essential inhibitory role playe d by three positive charges on this segment. The lack of prepulse-indu ced current facilitation recorded with some truncated forms of the bet a(2) subunit suggests the existence of an additional inhibitory sequen ce in the beta(2) subunit.