I. Beau et al., THE BASOLATERAL LOCALIZATION SIGNAL OF THE FOLLICLE-STIMULATING-HORMONE RECEPTOR, The Journal of biological chemistry, 273(29), 1998, pp. 18610-18616
The follicle-stimulating hormone receptor (FSHR) is physiologically lo
calized in the basolateral compartment of the membrane of Sertoli cell
s. This localization is also observed when the receptor is experimenta
lly expressed in Madin-Darby canine kidney cells. We thus used in vitr
o mutagenesis and transfection into these polarized cells to delineate
the basolateral localization signal of the receptor. The signal was l
ocalized in the C-terminal tail of the intracellular domain (amino aci
ds 678-691) at a marked distance of the membrane. Mutation of individu
al amino acids highlighted the importance of Tyr(684) and Leu(689). Th
e 14-amino acid sequence was grafted onto the p75 neurotrophin recepto
r and redirected this apical protein to the basolateral cell membrane
compartment, Deletion of amino acids 677-695 did not modify the intern
alization of the FSHR, showing that the basolateral localization signa
l of the FSHR is not colinear with its internalization signal.