P. Andersson et al., HETERONUCLEAR CORRELATION EXPERIMENTS FOR THE DETERMINATION OF ONE-BOND COUPLING-CONSTANTS, Journal of biomolecular NMR, 11(4), 1998, pp. 445-450
Spin-state selective experiments, HSQC-alpha/beta and CT-HMQC-alpha/be
ta, are proposed for the simple and rapid measurement of scalar one-bo
nd coupling constants in two-dimensional, H-1-detected N-15-H-1 or C-1
3-H-1 correlation experiments based on HSQC and HMQC schemes. Pairs of
subspectra are obtained, containing either the high-field or the low-
field component of the doublet representing the one-bond coupling cons
tant. The subspectral editing procedure retains the full sensitivity o
f HSQC and HMQC spectra recorded without heteronuclear decoupling duri
ng data acquisition, with a spectral resolution similar to that of dec
oupled spectra.