HETERONUCLEAR CORRELATION EXPERIMENTS FOR THE DETERMINATION OF ONE-BOND COUPLING-CONSTANTS

Spin-state selective experiments, HSQC-alpha/beta and CT-HMQC-alpha/be ta, are proposed for the simple and rapid measurement of scalar one-bo nd coupling constants in two-dimensional, H-1-detected N-15-H-1 or C-1 3-H-1 correlation experiments based on HSQC and HMQC schemes. Pairs of subspectra are obtained, containing either the high-field or the low- field component of the doublet representing the one-bond coupling cons tant. The subspectral editing procedure retains the full sensitivity o f HSQC and HMQC spectra recorded without heteronuclear decoupling duri ng data acquisition, with a spectral resolution similar to that of dec oupled spectra.