Tj. Hollmann et al., CLONING, EXPRESSION, SEQUENCE DETERMINATION, AND CHROMOSOME LOCALIZATION OF THE MOUSE COMPLEMENT C3A ANAPHYLATOXIN RECEPTOR GENE, Molecular immunology, 35(3), 1998, pp. 137-148
The complement C3a anaphylatoxin receptor (C3aR) is a seven-transmembr
ane G-protein coupled chemoattractant receptor that on binding the C3a
peptide ligand mediates numerous cellular responses, including histam
ine release from mast cells, smooth muscle contraction, and the direct
ed migration of eosinophils. To delineate the murine C3aR coding seque
nce, gene structure, 5'-flanking region, and chromosome location, cDNA
and genomic clones encoding the mouse C3a receptor were isolated, cha
racterized, and used in fluorescence in situ hybridization experiments
. The results from this study indicate that the murine C3a receptor st
ructural gene is a single copy gene of approximately 8 kb comprised of
2 exons which are separated by a large intervening intron of 4724 bp.
The first exon encodes 97 bp of 5'-untranslated sequence. Exon 2 enco
des the remaining 8 bp of 5'-untranslated sequence and the entire codi
ng and 3'-untranslated sequences. This genomic organization is typical
of most other chemoattractant receptor genes in that the entire codin
g sequence is contained on a single exon. The human and mouse C3a rece
ptor genes were localized to syntenic chromosomal bands 12q13.2-3 and
6F1, respectively. No other seven-transmembrane receptor genes, to dat
e, have been localized to these chromosomal regions. Primer extension
experiments using mouse macrophage RNA indicated a single transcriptio
nal initiation site. Sequence analysis 5' of the transcriptional site
indicated a TATA-less promoter with possible cis-acting motifs that ma
y regulate C3a receptor gene expression. These included the recognitio
n sequence for the nuclear transcription factor SP1 and the phorbol es
ter response sequence which binds the Fos/Jun heteromeric transcriptio
n factor API. (C) 1998 Elsevier Science Ltd. All rights reserved.