The CD45 exon usage pattern of various CD8(+) and CD4(+) T cell lines
was studied. By using the reverse transcription-polymerase chain react
ion (RT/PCR) and Southern analysis with exon specific or exon junction
probes, we showed that all of the cytotoxic T cell lines and the majo
rity of the helper T cells expressed the 789 isoform as a major splice
variant. Expression of the splice product lacking exons 4-7 (isoform
89) was not as ubiquitous. All Th lines produced mRNA encoding this is
oform, but in only three of the Tc lines was the 89 isoform detectable
by RT/PCR. RNase protection assays with RNA isolated from normal CD8(
+) splenic cells demonstrated the 89 splice product was present in low
abundance. The relative abundance of the different isoforms in the th
ymic lymphoma, BW5147, was determined through RNase protection analysi
s. The 789 isoform predominates, representing approximately 75% of the
CD45 mRNA whereas the 89 form constitutes about 24%. In addition: an
isoform lacking exons 4-8 (isoform 9) also was detected and comprises
approximately 1% of the total CD45 mRNA in this cell line. Finally: th
ese studies demonstrate that exon 10 is also used as an alternatively
spliced exon. (C) 1998 Elsevier Science Ltd. All rights reserved.