Mc. Carson et al., CONFIRMATION OF MULTIPLE TETRACYCLINE RESIDUES IN MILK AND OXYTETRACYCLINE IN SHRIMP BY LIQUID-CHROMATOGRAPHY PARTICLE-BEAM MASS-SPECTROMETRY, Journal of chromatography B. Biomedical sciences and applications, 712(1-2), 1998, pp. 113-128
Citations number
20
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
A confirmation procedure is described for detection of residues of six
tetracyclines in bovine milk, and oxytetracycline in shrimp. Residues
are extracted from milk or shrimp tissue using metal chelate affinity
chromatography. The extracts are desalted, further concentrated using
polymeric solid-phase extraction, and chromatographed on a polymeric
reversed-phase column. Analysis is by methane negative ion chemical io
nization on a quadrupole mass spectrometer using a particle beam inter
face. Data are acquired in partial scan mode, monitoring from m/z 378
to m/z 480. The procedure was validated with control milk and shrimp,
fortified milk (30 ng/ml) and shrimp (100 ng/g), and milk and tissue f
rom animals treated with the drugs. Published by Elsevier Science B.V.