CHIRAL CHROMATOGRAPHIC METHOD TO DETERMINE THE ENANTIOMERS OF HALOFANTRINE AND ITS MAIN CHIRAL DESBUTYL METABOLITE IN ERYTHROCYTES

Authors
GORICHON E MARTIN C BANGCHANG KN KARBWANG J THUILLIER A FARINOTTI R GIMENEZ F
Citation
E. Gorichon et al., CHIRAL CHROMATOGRAPHIC METHOD TO DETERMINE THE ENANTIOMERS OF HALOFANTRINE AND ITS MAIN CHIRAL DESBUTYL METABOLITE IN ERYTHROCYTES, Journal of chromatography B. Biomedical sciences and applications, 712(1-2), 1998, pp. 259-262
Citations number
17
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273 → ACNP
Volume
712
Issue
1-2
Year of publication
1998
Pages
259 - 262
Database
ISI
SICI code
0378-4347(1998)712:1-2<259:CCMTDT>2.0.ZU;2-P
Abstract
We describe a direct liquid chromatographic method with spectrofluorim etric detection to quantify the two enantiomers of halofantrine and th e two enantiomers of its main chiral N-monodesbutylated metabolite in erythrocyte pellets. The method involves a Chiralpak AD column and a r apid one-step extraction procedure with acetonitrile. The method was v alidated for the four enantiomers within the range 0-1000 ng/ml. The a bsence of stereoconversion was studied in samples stored frozen for up to eight months. The optical rotation of the halofantrine and metabol ite enantiomers was determined after separation on a semi-preparative Chiralcel OD column with polarimetric detection. (C) 1998 Elsevier Sci ence B.V. All rights reserved.