SALT-STEERED PARTITIONING OF PROTEINS IN POLYMERIC 2-PHASE SYSTEMS BASED ON N,N-DIMETHYLFORMAMIDE

Authors
TRUUST H JOHANSSON G
Citation
H. Truust et G. Johansson, SALT-STEERED PARTITIONING OF PROTEINS IN POLYMERIC 2-PHASE SYSTEMS BASED ON N,N-DIMETHYLFORMAMIDE, Journal of chromatography B. Biomedical sciences and applications, 711(1-2), 1998, pp. 117-126
Citations number
12
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273 → ACNP
Volume
711
Issue
1-2
Year of publication
1998
Pages
117 - 126
Database
ISI
SICI code
0378-4347(1998)711:1-2<117:SPOPIP>2.0.ZU;2-3
Abstract
The polymeric two-phase (liquid-liquid) system, based on N,N-dimethylf ormamide (DMFA) and the two polymers Ficoll and poly(ethylene glycol), has been investigated. The partitioning of the water-insoluble protei n fraction, zein (from corn), was affected by the addition of various salts that were soluble in DMFA. The effect of the salts has been corr elated to their partition coefficients. The data have been fitted to a theory for partitioning of polyelectrolytes in aqueous two-phase syst ems. The two-phase system has been used for partial fractionation of z ein by counter-current distribution. (C) 1998 Elsevier Science B.V. Al l rights reserved.