ENZYME-RELEASE FROM HEAT-STRESSED CELL-MEMBRANES AS A FUNCTION OF HYDROPHOBICITY EVALUATED BY USING AQUEOUS 2-PHASE SYSTEMS

Authors
UMAKOSHI H KUBOI R
Citation
H. Umakoshi et R. Kuboi, ENZYME-RELEASE FROM HEAT-STRESSED CELL-MEMBRANES AS A FUNCTION OF HYDROPHOBICITY EVALUATED BY USING AQUEOUS 2-PHASE SYSTEMS, Journal of chromatography B. Biomedical sciences and applications, 711(1-2), 1998, pp. 217-222
Citations number
29
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273 → ACNP
Volume
711
Issue
1-2
Year of publication
1998
Pages
217 - 222
Database
ISI
SICI code
0378-4347(1998)711:1-2<217:EFHCAA>2.0.ZU;2-Y
Abstract
The release behavior of a periplasmic enzyme, acid phosphatase, from h eat-stressed Escherichia coli cells was characterized by using kinetic analyses when the cells were treated by Triton X-100-EDTA. The hydrop hobicity of the cell surface and the release-rate of the enzyme were n ot influenced by heat treatment at temperatures between 30 and 50 degr ees C. However. these values varied above 55 degrees C. The release-ra te constants were found to correspond to the net and local hydrophobic ity of the outer membrane surface, evaluated by aqueous two-phase part itioning. (C) 1998 Elsevier Science B.V. All rights reserved.