FRACTIONATION OF WHEAT GLIADINS BY COUNTERCURRENT DISTRIBUTION USING AN ORGANIC 2-PHASE SYSTEM

Authors
TRUUST H JOHANSSON G
Citation
H. Truust et G. Johansson, FRACTIONATION OF WHEAT GLIADINS BY COUNTERCURRENT DISTRIBUTION USING AN ORGANIC 2-PHASE SYSTEM, Journal of chromatography B. Biomedical sciences and applications, 711(1-2), 1998, pp. 245-254
Citations number
21
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273 → ACNP
Volume
711
Issue
1-2
Year of publication
1998
Pages
245 - 254
Database
ISI
SICI code
0378-4347(1998)711:1-2<245:FOWGBC>2.0.ZU;2-2
Abstract
A liquid-liquid two-phase system based on N,N-dimethylformamide and th e two polymers, poly(ethyleneglycol) and Ficoll, useful for partitioni ng of hydrophobic proteins, has been developed. The system has been ap plied to a counter-current distribution process in 56 steps for analys ing the heterogeneity of proteins extracted with N,N-dimethylformamide from wheat flour. The counter-current distribution patterns of protei ns, extracted from eight kinds of wheat, have been analysed. The minim um number of hypothetical proteins necessary to describe the patterns was found to be seven. The relative amount of these hypothetical compo nents varied among the wheats. (C) 1998 Elsevier Science B.V. All righ ts reserved.