VARIATION OF PENICILLIN ACYLASE PARTITION-COEFFICIENT WITH PHASE VOLUME RATIO IN POLY(ETHYLENE GLYCOL) SODIUM-CITRATE AQUEOUS 2-PHASE SYSTEMS

Authors
MARCOS JC FONSECA LP RAMALHO MT CABRAL JMS
Citation
Jc. Marcos et al., VARIATION OF PENICILLIN ACYLASE PARTITION-COEFFICIENT WITH PHASE VOLUME RATIO IN POLY(ETHYLENE GLYCOL) SODIUM-CITRATE AQUEOUS 2-PHASE SYSTEMS, Journal of chromatography B. Biomedical sciences and applications, 711(1-2), 1998, pp. 295-299
Citations number
8
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273 → ACNP
Volume
711
Issue
1-2
Year of publication
1998
Pages
295 - 299
Database
ISI
SICI code
0378-4347(1998)711:1-2<295:VOPAPW>2.0.ZU;2-X
Abstract
The influence of phase volume ratio on partition and purification of p enicillin acylase from Escherichia coli on poly(ethylene glycol)-sodiu m citrate aqueous two-phase systems was studied. In PEG 1000 systems b oth partition coefficients of the enzyme and total protein increased w ith decreasing phase volume ratio. However, in PEG 3350 containing NaC l, penicillin acylase follows a reverse trend, while total protein beh aves in the same way. Implications for protein purification designs ar e discussed. (C) 1998 Elsevier Science B.V. All rights reserved.