INFLUENCE OF BILIARY OBSTRUCTION ON NEUTROPHIL CHEMOTAXIS

The effect of obstructive jaundice on neutrophil chemotactic function was investigated, with a potent chemotactic factor, IL-8 (recombinant rat GRO-beta), in rats that received 7-day bile duct ligation. Carrage enin or IL-8 was injected into a preformed air pouch, and exudate was collected 4 h later for measurement of myeloperoxidase activity. In vi tro chemotaxis of peripheral neutrophils to IL-8 was evaluated by a mo dified Boyden chamber method. Both carrageenin and IL-8 induced signif icantly pronounced intra-air pouch neutrophil recruitment in the bile duct-ligated group compared with a sham-ligated group. In vitro neutro phil chemotaxis was significantly increased in the bile duct-ligated g roup compared with the sham-ligated group. The present experimental mo del suggests enhanced neutrophil chemotaxis to IL-8 in obstructive jau ndice.