PRODUCTION OF ANTISERUM FOR SENSITIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY OF 3-CARBOXY-4-METHYL-5-PROPYL-2-FURANPROPANOIC ACID BY CHEMILUMINESCENCE

To obtain a specific antiserum for use in enzyme-linked immunosorbent assay (ELISA) of 3-carboxy-4-methyl-5-propyl-2 -furanpropanoic acid (C MPF), we prepared a hapten-carrier conjugate in which the CMPF hapten was linked to a carrier protein through the 5-(1-hydrazopropyl) group. The antisera raised against this antigen in guinea pigs had excellent -specificity for CMPF, showing little cross-reactivity with closely re lated compounds and no significant cross-reactivities with other furan compounds. The results indicated that a specific antiserum to CMPF co uld be produced by an antigen whose CMPF moiety is linked to a carrier protein through a position remote from the inherent functional groups . A standard curve of CMPF by ELISA using a chemiluminescence system s howed a high sensitivity and a linearity in the range bf 5-100 ng/mL.