ELUCIDATION OF THE COMBINING SITE OF COCCINIA-INDICA AGGLUTININ (CIA)BY THERMODYNAMIC ANALYSES OF ITS LIGAND-BINDING

Authors
SANADI AR ANANTHRAM V SUROLIA A
Citation
Ar. Sanadi et al., ELUCIDATION OF THE COMBINING SITE OF COCCINIA-INDICA AGGLUTININ (CIA)BY THERMODYNAMIC ANALYSES OF ITS LIGAND-BINDING, Pure and applied chemistry, 70(3), 1998, pp. 677-686
Citations number
17
Categorie Soggetti
Chemistry
Journal title
Pure and applied chemistry → ACNP
ISSN journal
00334545
Volume
70
Issue
3
Year of publication
1998
Pages
677 - 686
Database
ISI
SICI code
0033-4545(1998)70:3<677:EOTCSO>2.0.ZU;2-J
Abstract
Analyses of the ligand size dependence of the fluorescence spectra of CIA together with the thermodynamic parameters for the lectin reveal t hat its combining site spans the tetrasaccharide chitotetraose. Moreov er the fourth sugar residue of chitoligosaccharide is proximal to a hi ghly fluorescent tryptophan.