FUSARIUM PROLIFERATUM - INDUCTION AND INTRACELLULAR LOCATION OF A LIPOXYGENASE

The fungus Fusarium proliferatum was grown in a soya oil- and glucose- supplemented medium. Induction of lipoxygenase activity (13-fold) in a n exact of Fusarium biomass has been observed at day four on soya oil culture medium. A band at 232 kDa was detected using a specific lipoxy genase stain combined with native PAGE. The method of fungal homogenat e obtained has been checked via subcellular marker enzymes activities. succinate dehydrogenase (SDH) for mitochondrial fraction and NADH-cyt ochrome C reductase (NADH-cyt. C) for the microsomal fraction. Protopl asts production and disintegration followed by subcellular fractionati on using differential centrifugation was the best method to recover pu re fractions. The results of subcellular fractionation showed that lip oxygenase is mainly distributed among microsomal and mitochondrial fun ctions with a high enrichment of specific activity in the microsomal f raction. (C) 1998 Elsevier Science Inc.