2-DEOXYGLUCOSE TRANSPORT BY THE FROG SARTORIUS - EFFECTS OF ELECTRICAL-STIMULATION AND N-CARBOBENZOXY-GLYCYL-L-PHENYLALANINAMIDE

1. Studies characterizing glucose transport in the frog sartorius were performed. 2. For nonstimulated and stimulated muscles, intracellular 2-deoxyglucose exceeded 2-deoxyglucose-6-phosphate at 15 min, showed little further increase, and was maintained below the extracellular co ncentration for 2 hr. 3. Accumulated 2-deoxyglucose-6-phosphate did no t inhibit glucose transport-4. Unlike in adipocytes, basal and stimula ted 2-deoxyglucose transport showed no difference in sensitivity to N- carbobenzoxy-glycyl-L-phenylalaninamide. 5. Phenylarsine oxide blocked contraction-enhanced 2-deoxyglucose uptake. 6. These results suggest that the glucose transporter of the sartorius exhibits auto-regulation , and that basal transport is not regulated by the same process as in adipocytes.