NONRADIOACTIVE SINGLE-STRAND CONFORMATION POLYMORPHISM (SSCP) ANALYSIS OF EXON-11 OF THE CFTR GENE USING THE PHARMACIA PHASTSYSTEM

Authors
CORMIERDAIRE V CLAVEL C POLETTE M DOCO M BOUTTERIN MC BINNINGER I BIREMBAUT P
Citation
V. Cormierdaire et al., NONRADIOACTIVE SINGLE-STRAND CONFORMATION POLYMORPHISM (SSCP) ANALYSIS OF EXON-11 OF THE CFTR GENE USING THE PHARMACIA PHASTSYSTEM, Pathologie et biologie, 41(8), 1993, pp. 713-715
Citations number
11
Categorie Soggetti
Pathology
Journal title
Pathologie et biologie → ACNP
ISSN journal
03698114
Volume
41
Issue
8
Year of publication
1993
Pages
713 - 715
Database
ISI
SICI code
0369-8114(1993)41:8<713:NSCP(A>2.0.ZU;2-L
Abstract
The authors report here the use of the PhastSystem (Pharmacia) to perf orm the single strand conformation polymorphism analysis of polymerase chain reaction products of the exon 11 of the CFTR gene. It provides a rapid (2 hours) safe and reliable technique for the development of c arrier testing for individuals or couples with a family history of cys tic fibrosis.