ALDOSTERONE OVERPRODUCTION AND CYP11B2 MESSENGER-RNA OVEREXPRESSION IN VESSELS OF SPONTANEOUSLY HYPERTENSIVE RATS

Authors
WU PS GUO ZG ZHANG YH LIU YL LIANG XW ZHANG RH LAI WY TAKEDA Y ISAMU M TAKEDA R
Citation
Ps. Wu et al., ALDOSTERONE OVERPRODUCTION AND CYP11B2 MESSENGER-RNA OVEREXPRESSION IN VESSELS OF SPONTANEOUSLY HYPERTENSIVE RATS, Hormone research, 50(1), 1998, pp. 28-31
Citations number
20
Categorie Soggetti
Endocrynology & Metabolism
Journal title
Hormone research → ACNP
ISSN journal
03010163
Volume
50
Issue
1
Year of publication
1998
Pages
28 - 31
Database
ISI
SICI code
0301-0163(1998)50:1<28:AOACMO>2.0.ZU;2-9
Abstract
Aldosterone is synthesized in extra-adrenal tissues such as blood vess els and brain. Damage to blood vessels could play a crucial role in pe rpetuating hypertension. In this study, we determined both aldosterone production and aldosterone synthase gene-CYP11B2 mRNA expression in v essels of spontaneously hypertensive rats (SHR). The results showed th at aldosterone was overproduced in the vasculature of SHR, by means of ex vivo mesenteric artery perfusion, HPLC, and RIA, and that CYP11B2 mRNA expression was upregulated in aortas of SHR, confirmed by RT-PCR and Southern blot.