Gb. Karkanias et al., EFFECTS OF DIABETES AND ESTRADIOL ON NOREPINEPHRINE RELEASE IN FEMALERAT HYPOTHALAMUS, PREOPTIC AREA AND CORTEX, Neuroendocrinology, 68(1), 1998, pp. 30-36
These studies determined whether diabetes and estradiol treatment alte
red norepinephrine (NE) release from hypothalamus, preoptic area (POA)
, and cortical slices from ovariectomized (OVX) female rats. Animals w
ere sacrificed 12 days after the onset of streptozotocin-induced diabe
tes and 48 h following vehicle or estradiol injection, Brain slices we
re preloaded with H-3-NE, and release was evoked twice (S1 and S2) by
electrical stimulation. Diabetes increased hypothalamic NE release dur
ing S1 regardless of the administration of vehicle or estradiol. Neith
er estradiol treatment nor diabetes alone affected NE release during S
2 in the hypothalamus or POA. Estradiol treatment elevated NE release
in the POA during S2 but only in diabetic animals. Moreover, estradiol
elevated cortical NE release during S2 regardless of the presence or
absence of disease. We also examined whether alpha(2)-adrenoceptor reg
ulation of NE release was influenced by diabetes or hormone treatment.
Enhancement of NE release by alpha(2)-adrenoceptor antagonism was evi
dent in all 3 brain regions. However, alpha(2)-adrenoceptor regulation
of NE release was unaffected by diabetes and hormone treatment. These
findings suggest that diabetes alters NE release in the hypothalamus/
POA of female rats. Additionally, this work identifies a novel action
of estradiol to enhance stimulated NE release in the cortex of female
rats.