NUCLEAR-RELAXATION ANALYSIS OF THE XENOBIOTIC-RECEPTOR (DNA OR PLASMATIC PROTEIN) RECOGNITION PROCESS

The study of interactions of xenobiotics with macromolecular receptors is very important for understanding the chemical behaviour of xenobio tic compounds in the biological organisms. The xenobiotic molecules ar e able to affect the natural activities of biological receptor such as DNA or plasmatic protein. In fact the modification of the conformatio n of DNA or plasmatic protein, induced by interaction with xenobiotic molecules, can determine profound alterations of the normal biochemica l activity. In this study a method based on proton NMR selective and n on-selective spin-lattice relaxation rate measurements and their depen dence on temperature for analyzing the ability of ligand to interact w ith receptor is used. The NMR parameters are a weighted average betwee n the free and bound to xenobiotic environments.