Jl. Goodall et Sw. Peretti, DYNAMIC MODELING OF META-NITROBENZOATE AND PARA-NITROBENZOATE METABOLISM BY A MIXED CO-IMMOBILIZED CULTURE OF COMAMONAS SPP. JS46 AND JS47, Biotechnology and bioengineering, 59(4), 1998, pp. 507-516
A model describing the transient activity of a mixed immobilized cultu
re of Comamonas spp. JS46 and JS47 growing on mixed substrates is pres
ented. The transient periods considered are those following changes in
the feed carbon source, which alternated between meta- and para-nitro
benzoate, The feed profile alternately starved one of the species in t
he mixed culture. The response of the system, as quantified by the rea
ctor effluent substrate concentrations, is dictated by the activity of
the biomass and the appropriate biochemical pathway. As detailed mech
anistic pathway information is not available, respirometry has been us
ed to characterize both facets of activity. Two parameters were introd
uced: psi representing pathway activity and Gamma representing biomass
activity; a detailed description of the analysis is included. The mod
el is compared to experimental investigation of the system and describ
es the reactor response well. The agreement between model and experime
nt suggests the usefulness of oxygen kinetics as global measurements t
o describe complex systems when mechanistic detail is not available. (
C) 1998 John Wiley & Sons, Inc.