A NEW SERIES OF CYCLIC AMINO-ACIDS AS INHIBITORS OF S-ADENOSYL L-METHIONINE SYNTHETASE

Optically active 3-amino-3-(tetrahydrofuranyl) carboxylic acid, 3-amin o-3 -(tetrahydrothienyl) carboxylic acid and their corresponding six m embered ring analogues have been synthesised and examined as potential inhibitors of the enzyme S-adenosylmethionine (AdoMet) synthetase.The kinetic behaviour of these compounds was studied using recombinant ra t liver AdoMet synthetase (alpha-isoform) fractionated from E. coli tr ansformed with the plasmid pSSRL-T7N. All the compounds tested were co mpetitive inhibitors of the enzyme with respect to L-methionine. (C) 1 998 Elsevier Science Ltd. All rights reserved.