HOMOLOGY-BASED FOLD PREDICTIONS FOR MYCOPLASMA-GENITALIUM PROTEINS

Homology search techniques based on the iterative PSI-BLAST method in combination with various filters for low sequence complexity are appli ed to assign folds to all Mycoplasma genitalium proteins. The resultin g procedure (implemented as a web server) is able to predict at least one domain in 37% of these proteins automatically, with an estimated a ccuracy higher than 98%. Taking structural features such as coiled coi l or transmembrane regions aside, folds can be assigned to more than h alf of the globular proteins in a bacterium just by iterative sequence comparison. (C) 1998 Academic Press.