DETERMINATION OF ANTIIDIOTYPE ANTIBODIES BY SURFACE-PLASMON RESONANCE

A method based on surface plasmon resonance has been used to detect an d to quantitate antibodies against other antibodies. This method has b een applied to the detection of syngeneic antibodies. Concentrations a s low as 0.3 mu g/ml could be detected even when the antibodies were p resent in undiluted serum. Quantitation of both monoclonal and polyclo nal antibodies could be performed. Anti-idiotype antibodies could be d etected equally well against an IgM molecule derived directly from the B cell tumour or against a chimeric protein composed of the same idio typic determinants embedded in a human IgG molecule. Surface plasmon r esonance will be the method of choice for the detection of antibody re sponses in humans vaccinated against idiotypic determinants of their o wn tumour. (C) 1998 Elsevier Science B.V. All rights reserved.