EXPRESSION OF AN ADENYLATE-CYCLASE GENE OF ANABAENA-CYLINDRICA IN THECYANOBACTERIUM ANABAENA SP. STRAIN PCC-7120

Authors
KATAYAMA M OHMORI M
Citation
M. Katayama et M. Ohmori, EXPRESSION OF AN ADENYLATE-CYCLASE GENE OF ANABAENA-CYLINDRICA IN THECYANOBACTERIUM ANABAENA SP. STRAIN PCC-7120, Plant and Cell Physiology, 39(7), 1998, pp. 786-789
Citations number
12
Categorie Soggetti
Plant Sciences","Cell Biology
Journal title
Plant and Cell Physiology → ACNP
ISSN journal
00320781
Volume
39
Issue
7
Year of publication
1998
Pages
786 - 789
Database
ISI
SICI code
0032-0781(1998)39:7<786:EOAAGO>2.0.ZU;2-K
Abstract
A transformant of Anabaena 7120 was made by introducing a plasmid that includes an adenylate cyclase gene of Anabaena cylindrica. Expression of this gene was driven by the bacterial tac promoter. Transformants accumulate cAMP 170 fold higher than the concentration in the parental strain. The transformation resulted in the fragmentation of filaments in both nitrogen-replete and nitrogen-free media. It was suggested th at this fragmentation caused the inhibition of growth under nitrogen-f ixing conditions.