GRAPE MICROSATELLITE MARKERS - SIZING OF DNA ALLELES AND GENOTYPE ANALYSIS OF SOME GRAPEVINE CULTIVARS

Capillary electrophoresis fluorescence-based technology and fragment s izing software were applied in order to type grape cultivars at micros atellite loci described by THOMAS and SCOTT (1993) and BOWERs et al. ( 1996). Whereas the data set was closely consistent with already known genotypes for the same cultivars, estimates of allele length differed remarkably from those reported. The pedigree of the Riesling crosses B acchus, Optima, Muller-Thurgau, Scheurebe, Ehrenfelser, Kerner, Rotber ger, Rieslaner and Incrocio Manzoni 6.0.13 were investigated, and Mend elian inheritance of automatically sized alleles proved to be easily s corable. In addition, ancient cultivars of the vine-growing area of Tr entino were characterized at microsatellite loci. Genotypes of Marzemi no, Teroldego, Lagrein, Lambrusco a foglia frastagliata and Groppello gentile proved to share more than 50 % of alleles.