PROTECTIVE EFFECTS OF CHLOROGENIC ACID, CURCUMIN AND BETA-CAROTENE AGAINST GAMMA-RADIATION-INDUCED IN-VIVO CHROMOSOMAL DAMAGE

The mouse bone marrow micronucleus test was carried out to evaluate th e possible role of the dietary constituents chlorogenic acid (CGA), cu rcumin (CR) and beta-carotene (BC) in modulating the in vivo chromosom al damage induced by gamma-radiation. The results obtained suggest tha t oral administration of CGA (50, 100 and 200 mg/kg b.w.), CR (5, 10 a nd 20 mg/kg b.w.) and BC (0.5 and 2.5 mg/kg b.w.) to mice can signific antly reduce the frequencies of micronucleated polychromatic erythrocy tes (Mn PCEs) induced by whole body exposure to gamma-radiation (1.15 Gy; 0.05 Gy/s). With CGA and CR, this effect was observed after a sing le administration either 2 h before or immediately after irradiation. However, with BC a 7-day feeding before irradiation was necessary to o btain a significant reduction in the incidence of Mn PCEs. The protect ive effects of CGA, CR and BC were observed in bone marrow cells sampl ed 24, 30 and 48 h after exposure to radiation.