CLONING AND SEQUENCING OF CDNA-ENCODING RIBONUCLEASE F1 FROM FUSARIUM-MONILIFORME

Two cDNA clones encoding ribonuclease F1 (EC 3. 1. 27. 3) have been is olated using a probe prepared by polymerase chain reaction with primer s designed on the basis of amino acid sequence of the enzyme. They der ived probably from the same gene and contained 393-base pair open read ing frame encoding 131 amino acid residues (Mr 13,606) including a put ative 25-residue signal peptide. The sequences of 43-base pair 5'-untr anslated region and 125-base pair 3'-untranslated region including a p oly(A) tail of 25 nucleotides were also elucidated. Homology analyses showed that cDNA for ribonuclease F1 has 65% homology to that for ribo nuclease T1 in the coding region. At the preprotein level, they share 53% homology.