GLUTATHIONE OXIDASE-LIKE ACTIVITY OF GLASS-BEADS, SILICA-GEL AND ANION-EXCHANGE RESIN MODIFIED WITH COBALT(III)-TETRAKIS(4-CARBOXYPHENYL)PORPHINE

Silica gel and glass beads were modified by using acid chloride of met al-tetrakis(4-carboxyphenyl)porphine (M-TCPP) through a peptide bond, and an anion-exchange resin with M-TCPP by ion-exchange reaction and p hysical adsorption. The carriers modified with Co3+-TCPP proved to acc elerate the redox reaction which is catalyzed by glutathione oxidase ( GSHOx), while those modified with Mn3+-TCPP exhibited no activity. For mation of GS-SG and hydrogen peroxide was confirmed by means of mass s pectroscopy and colored reaction, respectively. The silica gel modifie d with Co3+-TCPP exhibited the strongest activity among the tested car riers, and was expected to be useful practically as a solid catalyst f or the determination of glutathione. (C) 1998 Elsevier Science B.V. Al l rights reserved.