DEVELOPMENT OF FLOW-INJECTION LIPOSOME IMMUNOANALYSIS (FILIA) FOR IMAZETHAPYR

Imazethapyr is the herbicide developed for use in leguminous crops. In this study, flow-injection liposome immunoanalysis (FILIA) has been s hown to be capable of measuring imazethapyr in a buffered solution wit h a detection limit of 0.1 ppb through the optimization process. Prote in A coated glass beads covalently conjugated with antibody were conta ined in a glass column, and this column was used as an immunoreactor. Liposomes which encapsulated a fluorescent dye, sulforhodamine B (SRB) or carboxyfluorescein (CF), generated the analytical signal. By loadi ng larger volumes of sample onto the column, it was shown that the det ection limit could be lowered. Liposomes containing carboxyfluorescein gave more sensitive response and a lower detection limit than those w ith sulforhodamine B. Also, improved response was obtained by using a smaller flow cell in the fluorescence detector due to the reduced dilu tion effect. (C) 1998 Elsevier Science B.V. All rights reserved.