M. Sagi et S. Herman, THE LEVELS OF NITRATE REDUCTASE AND MOCO IN ANNUAL RYEGRASS AS AFFECTED BY NITRATE AND AMMONIUM NUTRITION, PLANT SCI, 135(1), 1998, pp. 17-24
The influence of NH4+ And NO3- on the activity of nitrate reductase(NR
; EC 1. 6.6.1) and the level of the molybdenum cofactor (MoCo) was stu
died in annual ryegrass (Lolium multiflorum cv Westerwoldicum). Additi
on of 4.5 mM NH4+ to plants grown with NO3- Increased NR activity in t
he shoots, but decreased it in the root. Total MoCo increased in both
roots and shoots. Plants grown with only NH4+ increased their MoCo con
tent with N concentration in the medium, while NR activity remained at
constitutive levels. NR activity, total MoCo content, readily availab
le MoCo (determined without a heat pretreatment) and NO3- accumulation
increased in plant root and shoot within 1 day in NO3-. NH4+ triggere
d the increase of total and readily available MoCo content in shoot an
d root above the constitutive level after 1 day of induction. MoCo bio
synthesis may be regulated by NH4+ end NO3- or one of their common ass
imilation products. N-regulation of MoCo in the roots differs from the
shoots in that: (a) total root MoCo increased proportionally more tha
n NR in plants grown with NO3-, while total shoot MoCo and NR activity
levels were quite similar; and (b) The NH4+ induced increase in MoCo
was proportionally higher in roots than in shoots, suggesting a higher
demand of MoCo in the root for the biosynthesis of molybdo-enzymes ot
her than NR. (C) 1998 Elsevier Science Ireland Ltd. All rights reserve
d.