ENHANCEMENT OF ESTROGEN-INDUCED RENAL TUMORIGENESIS IN HAMSTERS BY DIETARY IRON

Iron participates in the generation of hydroxyl radicals by the iron-c atalyzed Fenton reaction. Its role in estrogen-induced carcinogenesis has been examined in this study by investigating the effects of iron c ontent of hamster diets on tumor induction by estradiol, The renal tum or incidence and number of tumor nodules in hamsters treated with estr adiol plus a diet enriched with iron (384 p.p.m. Fe as ferric citrate) for 5 months were 2- and 4-fold higher, respectively, than those obse rved in animals on an iron-poor diet plus estradiol (3.9 p.p,m. Fe, as ferric citrate). Tumor incidence and number of tumor nodules in estra diol-treated hamsters on the iron-deficient diet were not different fr om those of animals on a normal rodent chow No tumors were detected in hamsters treated only with the low or high iron diets. Total serum ir on was significantly increased in animals treated with the high iron d iet plus estradiol compared with the low iron diet plus estradiol grou p and the high and low iron controls. Estrogen treatment increased non -heme iron in liver of both high and low iron treatment groups and in kidney of the hamsters on the low iron diet. It is concluded that diet ary iron enrichment enhances the incidence and severity of estrogen-in duced tumor induction.