The mechanism of glucose oxidase action in breadmaking was investigate
d by studying the baking performance of glucose oxidase, the active in
gredient that it produced, and its effect on the rheological propertie
s of dough. Glucose oxidase improved the loaf volume of bread made by
45-, 70-, and 90-min fermentation processes. Although the increase in
loaf volume was significant, it was less than that obtained with an op
timum level of KBrO3. With the 90-min fermentation process, the crumb
grain of bread was similar for leaves oxidized with optimum levels of
glucose oxidase or KBrO3. The rheological properties of doughs contain
ing glucose oxidase and doughs containing no oxidant were compared. Do
ughs made with glucose oxidase had higher G' and G '' and lower tan de
lta values than doughs made without an oxidant. Hydrogen peroxide was
responsible for a drying effect in doughs. This drying effect of gluco
se oxidase was reduced significantly by incorporation of free radical
scavengers into the dough.