IMMOBILIZATION OF LIPASES ON CLAY, CELITE-545, DIETHYLAMINOETHYL-SEPHADEX, AND CARBOXYMETHYL-SEPHADEX AND THEIR INTERESTERIFICATION ACTIVITY

Authors
LEE KT AKOH CC
Citation
Kt. Lee et Cc. Akoh, IMMOBILIZATION OF LIPASES ON CLAY, CELITE-545, DIETHYLAMINOETHYL-SEPHADEX, AND CARBOXYMETHYL-SEPHADEX AND THEIR INTERESTERIFICATION ACTIVITY, Biotechnology techniques, 12(5), 1998, pp. 381-384
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
5
Year of publication
1998
Pages
381 - 384
Database
ISI
SICI code
0951-208X(1998)12:5<381:IOLOCC>2.0.ZU;2-7
Abstract
Lipases AK, PS (both from Pseudomonas so), and AY (from Candida rugosa ) were immobilized on clay, Celite 545, DEAE-Sephadex, or CM-Sephadex and used to synthesize structured lipids from tricaprylin and trilinol ein. Lipases AK and AY immobilized on Celite 545 had the highest inter esterification activity (0.11 and 0.35 units, respectively) while lipa se PS had the highest activity (0.18 units) when immobilized on CM-Sep hadex. During 72 h reaction, the highest product yields were obtained in Celite AK (224 mu mol), and CM PS (290 mu mol) after 48 h and Celit e AY (501 mu mol) after 24 h. Lipase AK immobilized on DEAE-Sephadex w as the most stable for repeated (5 times) operation.