CLONING AND SEQUENCING OF THE NRDF GENE OF CORYNEBACTERIUM AMMONIAGENES ATCC-6872 ENCODING THE FUNCTIONAL METALLO-COFACTOR OF THE MANGANESE-RIBONUCLEOTIDE REDUCTASE (MN-RRASE)

Authors
OEHLMANN W GRIEPENBURG U AULING G
Citation
W. Oehlmann et al., CLONING AND SEQUENCING OF THE NRDF GENE OF CORYNEBACTERIUM AMMONIAGENES ATCC-6872 ENCODING THE FUNCTIONAL METALLO-COFACTOR OF THE MANGANESE-RIBONUCLEOTIDE REDUCTASE (MN-RRASE), Biotechnology letters, 20(5), 1998, pp. 483-488
Citations number
20
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
20
Issue
5
Year of publication
1998
Pages
483 - 488
Database
ISI
SICI code
0141-5492(1998)20:5<483:CASOTN>2.0.ZU;2-Y
Abstract
Cloning of a 4.3 kb DNA fragment from Corynebacterium (formerly Brevib acterium) ammoniagenes ATCC 6872 allowed sequencing and homologous exp ression of the nrdF gene encoding the metallo-cofactor (CA2 protein) o f the Mn-RRase. Due to a multi copy effect transconjugants of C. ammon iagenes showed a sixfold increased resistance towards the radical-scav enger hydroxyurea (HU). The organic radical in the purified CA2 protei n generated a visible absorption at 437 rim, absent upon manganese-dep letion, and a distinct fluorescence at 513 nm. The two signals were ea ch quenched by HU.