TREATMENT OF CANDIDA-RUGOSA LIPASE WITH SHORT-CHAIN POLAR ORGANIC-SOLVENTS ENHANCES ITS HYDROLYTIC AND SYNTHETIC ACTIVITIES

Authors
CHAMORRO S SANCHEZMONTERO JM ALCANTARA AR SINISTERRA JV
Citation
S. Chamorro et al., TREATMENT OF CANDIDA-RUGOSA LIPASE WITH SHORT-CHAIN POLAR ORGANIC-SOLVENTS ENHANCES ITS HYDROLYTIC AND SYNTHETIC ACTIVITIES, Biotechnology letters, 20(5), 1998, pp. 499-505
Citations number
28
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
20
Issue
5
Year of publication
1998
Pages
499 - 505
Database
ISI
SICI code
0141-5492(1998)20:5<499:TOCLWS>2.0.ZU;2-A
Abstract
Following a simple and quick treatment based on dissolving the crude l ipase from Candida rugosa in different percentages (v/v) of several po lar organic solvents (methanol, ethanol, 1 and 2-propanol, 1 and 2-but anol and acetone) followed by dialysis, different preparations with en hanced activities were obtained. The opening of the lid covering the a ctive site is proposed as the reason for explaining the activity enhan cement, both in aqueous and anhydrous organic media.