FIBRIN GLUE AS A DELIVERY VEHICLE FOR AUTOLOGOUS UROTHELIAL CELL TRANSPLANTATION ONTO A PREFABRICATED POUCH

Purpose: The purpose of the study was to investigate how in vivo expan ded urothelium can be transplanted onto a prefabricated capsule-pouch for urinary reconstruction. Materials and Methods: Urothelial cells fr om 40 rats were harvested for culture, A tissue expander was used to i nduce a capsule-pouch on which the cell culture were reimplanted ten d ays later. As delivery vehicle we compared standard culture media and fibrin glue. Results: The histology demonstrated viable, multilayered clusters of urothelium cells only in the group using the fibrin glue d elivery vehicle. Conclusion: We conclude that cultured urothelial cell s can be successfully reimplanted onto a prefabricated capsule-pouch v ia fibrin glue, showing potential for urinary reconstructions.