A REPRODUCIBLE, SIMPLE AND SENSITIVE HIGH-PERFORMANCE CAPILLARY-ELECTROPHORESIS METHOD FOR SIMULTANEOUS DETERMINATION OF CAPREOMYCIN, OFLOXACIN AND PASINIAZIDE IN URINE

Separation and determination of capreomycin (Cp, ofloxacin (Oflx) and pasiniazide (Ipa) in urine by high-performance capillary electrophores is (HPCE) with 280 nm detection have been studied systematically. The calibration lines were linear in the range of 0.5 similar to 50 mg l(- 1), and the detection limits (S/N = 3) were 0.15, 0.20 and 0.10 mg l(- 1) for Cp, Oflx and Ipa, respectively. The recoveries for these materi als from urine were higher than 93.5%. The accuracy and intra- and int er-day reproducibility of Cp, Oflx and Ipa were determined with satisf actory results. This method was successfully used for determining Cp, Oflx and Ipa in human urine. (C) 1998 Elsevier Science B.V. All rights reserved.