QUANTITATIVE IMAGING MICROSCOPY FOR THE SENSITIVE DETECTION OF ADMINISTERED METAL-CONTAINING DRUGS IN SINGLE CELLS AND TISSUE-SLICES - A DEMONSTRATION USING PLATINUM-BASED CHEMOTHERAPEUTIC-AGENTS

Citation
Rj. Mauthe et al., QUANTITATIVE IMAGING MICROSCOPY FOR THE SENSITIVE DETECTION OF ADMINISTERED METAL-CONTAINING DRUGS IN SINGLE CELLS AND TISSUE-SLICES - A DEMONSTRATION USING PLATINUM-BASED CHEMOTHERAPEUTIC-AGENTS, Journal of pharmaceutical and biomedical analysis, 17(4-5), 1998, pp. 651-663
Citations number
31
Categorie Soggetti
Pharmacology & Pharmacy","Chemistry Analytical
ISSN journal
07317085
Volume
17
Issue
4-5
Year of publication
1998
Pages
651 - 663
Database
ISI
SICI code
0731-7085(1998)17:4-5<651:QIMFTS>2.0.ZU;2-#
Abstract
We describe the use of Nuclear microscopy (microbeam PIXE) for the qua ntitative micron scale analysis of platinum based chemotherapeutic age nts in individual cells and tissue slices. We demonstrate that microbe am PIXE has the sensitivity and accuracy to quantitatively measure the uptake of the chemotherapeutic agent cis-diamminedichloroplatinum(II) (cisplatin) and monitor other endogenous metal contents in single cel ls in a time- and dose-dependent fashion. Additionally, the technique can quantitatively image therapeutic levels of cisplatin and cisplatin analogs including diammine[1,1-cyclobutanedicarboxylato]platinum(II) (carboplatin) in tissues from an animal model. This quantitative imagi ng microscopy has general application for the sensitive measurement of metal containing drugs/compounds at the cellular level and allows the study of cellular distribution and mechanism of action related to tox ic response and cell function. (C) 1998 Elsevier Science B.V. All righ ts reserved.