QUANTITATIVE IMAGING MICROSCOPY FOR THE SENSITIVE DETECTION OF ADMINISTERED METAL-CONTAINING DRUGS IN SINGLE CELLS AND TISSUE-SLICES - A DEMONSTRATION USING PLATINUM-BASED CHEMOTHERAPEUTIC-AGENTS
Rj. Mauthe et al., QUANTITATIVE IMAGING MICROSCOPY FOR THE SENSITIVE DETECTION OF ADMINISTERED METAL-CONTAINING DRUGS IN SINGLE CELLS AND TISSUE-SLICES - A DEMONSTRATION USING PLATINUM-BASED CHEMOTHERAPEUTIC-AGENTS, Journal of pharmaceutical and biomedical analysis, 17(4-5), 1998, pp. 651-663
We describe the use of Nuclear microscopy (microbeam PIXE) for the qua
ntitative micron scale analysis of platinum based chemotherapeutic age
nts in individual cells and tissue slices. We demonstrate that microbe
am PIXE has the sensitivity and accuracy to quantitatively measure the
uptake of the chemotherapeutic agent cis-diamminedichloroplatinum(II)
(cisplatin) and monitor other endogenous metal contents in single cel
ls in a time- and dose-dependent fashion. Additionally, the technique
can quantitatively image therapeutic levels of cisplatin and cisplatin
analogs including diammine[1,1-cyclobutanedicarboxylato]platinum(II)
(carboplatin) in tissues from an animal model. This quantitative imagi
ng microscopy has general application for the sensitive measurement of
metal containing drugs/compounds at the cellular level and allows the
study of cellular distribution and mechanism of action related to tox
ic response and cell function. (C) 1998 Elsevier Science B.V. All righ
ts reserved.